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Development of a chimeric protein based on a proteomic approach for the serological diagnosis of human tegumentary leishmaniasis

Appl Microbiol Biotechnol. 2021-08; 
Guilherme Caetano Garcia, Ana Maria Ravena Severino Carvalho, Mariana Costa Duarte, Matheus Fernandes Costa E Silva, Fernanda Alvarenga Cardoso Medeiros, Eduardo Antonio Ferraz Coelho, Dênia Monteiro de Moura Franco, Denise Utsch Gonçalves, Tiago Antônio de Oliveira Mendes, Daniel Menezes-Souza
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Bacterial Expression … Gene-encoded chimeric protein was synthesized by a specialized biotechnology company, GenScript Company (New Jersey, USA). Electroporation of E. coli cells and expression, induction, and the purification of the recombinant chimeric protein (rChiP) was carried out as … Get A Quote

摘要

Leishmania braziliensis is responsible for most cases of human tegumentary leishmaniasis (HTL) and has caused a wide range of clinical manifestations, including cutaneous (CL) and mucosal leishmaniasis (ML). The diagnosis is based on criteria that consider epidemiological data, clinical findings, and laboratory tests and is hard to establish. For laboratory tests, none of the assays available can be considered gold standards for disease detection. In addition, the Montenegro skin test, essential to supporting infectologists in the clinical management of the disease, is no longer available in Brazil. Thus, the aim of this study was to develop new targets to be used in diagnostic tests for HTL. In the first step,... More

关键词

B-cell epitope mapping, Chimeric protein, Human tegumentary leishmaniasis, Immunodiagnosis, Immunoinformatics, Leishmania braziliensis, Proteomic