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RNase κ promotes robust piRNA production by generating 2',3'-cyclic phosphate-containing precursors

Nat Commun. 2021-07; 
Megumi Shigematsu, Takuya Kawamura, Keisuke Morichika, Natsuko Izumi, Takashi Kiuchi, Shozo Honda, Venetia Pliatsika, Ryuma Matsubara, Isidore Rigoutsos, Susumu Katsuma, Yukihide Tomari, Yohei Kirino
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Recombinant Antibody Expression … Anti-BmRNase κ antibody was prepared by immunizing the rabbits with a synthetic peptide (CEDLPFDEKNPPHSI; Genscript). The whole IgG was purified from the sera using Protein G Agarose (Sigma) to obtain the “BMK5263” anti-BmRNase κ antibody. … Get A Quote

摘要

In animal germlines, PIWI proteins and the associated PIWI-interacting RNAs (piRNAs) protect genome integrity by silencing transposons. Here we report the extensive sequence and quantitative correlations between 2',3'-cyclic phosphate-containing RNAs (cP-RNAs), identified using cP-RNA-seq, and piRNAs in the Bombyx germ cell line and mouse testes. The cP-RNAs containing 5'-phosphate (P-cP-RNAs) identified by P-cP-RNA-seq harbor highly consistent 5'-end positions as the piRNAs and are loaded onto PIWI protein, suggesting their direct utilization as piRNA precursors. We identified Bombyx RNase Kappa (BmRNase κ) as a mitochondria-associated endoribonuclease which produces cP-RNAs during piRNA biogenesis. BmRNase �... More

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