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Antigenic and immunogenic evaluation of permutations of soluble hepatitis C virus envelope protein E2 and E1 antigens

PLoS ONE. 2021-07; 
Jannick Prentoe, Christoph M Janitzek, Rodrigo Velázquez-Moctezuma, Louise Goksøyr, Rebecca W Olsen, Margherita Fanalista, Elias H Augestad, Susan Thrane, Anne F Pihl, Judith M Gottwein, Adam F Sander, Jens Bukh
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PCR Cloning and Subcloning … Constructs were generated using standard molecular cloning techniques with the exception of inverted sE1 sequence, the s1E sequence, which was synthesized de novo (Genscript), including the reintroduction of four N-linked glycosylation sites by the substitution V43T and … Get A Quote

摘要

Yearly, about 1.5 million people become chronically infected with hepatitis C virus (HCV) and for the 71 million with chronic HCV infection about 400,000 die from related morbidities, including liver cirrhosis and cancer. Effective treatments exist, but challenges including cost-of-treatment and wide-spread undiagnosed infection, necessitates the development of vaccines. Vaccines should induce neutralizing antibodies (NAbs) against the HCV envelope (E) transmembrane glycoprotein 2, E2, which partly depends on its interaction partner, E1, for folding. Here, we generated three soluble HCV envelope protein antigens with the transmembrane regions deleted (i.e., fused peptide backbones), termed sE1E2 (E1 followed by... More

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