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Antibodies Against the Clock Proteins Period and Cryptochrome Reveal the Neuronal Organization of the Circadian Clock in the Pea Aphid

Front Physiol. 2021-07; 
Francesca Sara Colizzi, Katharina Beer, Paolo Cuti, Peter Deppisch, David Martínez Torres, Taishi Yoshii, Charlotte Helfrich-Förster
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PCR Cloning and Subcloning … The corresponding DNA was synthesized after codon optimization for bacterial expression (GenScript, New Jersey, United States). The synthesized DNA was cloned into the pGEX-6p-1 vector (GE Healthcare, Chicago, United States) using the In-Fusion cloning method (Takara… Get A Quote

摘要

Circadian clocks prepare the organism to cyclic environmental changes in light, temperature, or food availability. Here, we characterized the master clock in the brain of a strongly photoperiodic insect, the aphid , immunohistochemically with antibodies against Period (PER), Cryptochrome (CRY1), and crab Pigment-Dispersing Hormone (PDH). The latter antibody detects all so far known PDHs and PDFs (Pigment-Dispersing Factors), which play a dominant role in the circadian system of many arthropods. We found that, under long days, PER and CRY are expressed in a rhythmic manner in three regions of the brain: the dorsal and lateral protocerebrum and the lamina. No staining was detected with anti-PDH, suggesting that... More

关键词

aphids, circadian clock, cryptochrome, hemiptera, insects, period, photoperiodism