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LINC00667 Promotes Progression of Esophageal Cancer Cells by Regulating miR-200b-3p/SLC2A3 Axis

Dig Dis Sci. 2021-07; 
Jindun Pan, Yunhong Zang
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Mutant Libraries … by GenScript (Nanjing, China). All the cells were grown to 80% confluence, and lipofectamine 2000 (Invitrogen, USA) was used for cell transfection. Besides, the full length and mutant of LINC00667 or 3′UTR of SLC2A3 were provided for luciferase assay by GenScript (Nanjing… Get A Quote

摘要

background: Recently, more and more evidence indicated that the long non-coding RNA was strictly related to the occurrence and progression of human cancers, including esophageal cancer (EC). We observed that LINC00667 was increased in EC, but the function of LINC00667 was unclear. Therefore, the function and potential molecular mechanism of LINC00667 in the progression of EC need to be further studied. methods: Quantitative real-time PCR was used to investigate the levels of LINC00667, miR-200b-3p, and SLC2A3. The levels of protein involved in cell cycle, cell apoptosis, epithelial-mesenchymal transition, as well as SLC2A3 were quantitatived by western blot. The role of LINC00667 in the proliferative, migratory... More

关键词

Apoptosis, Epithelial–mesenchymal transition, Esophageal cancer, SLC2A3, lncRNA, miR-200b-3p