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GenParts™ DNA Fragments> | … cerevisiae 22 , and lipaseB from Candida antarctica 53 were all synthesized by GenScript (Piscataway, NJ, USA). The obtained gene fragments of vaat, saat, atf1, atf1’, eth1, and lipaseB were inserted between the BtgZI and EcoRI sites in pMTL82151-P cat , generating pMTL-P … | Get A Quote |
Bioproduction of renewable chemicals is considered as an urgent solution for fossil energy crisis. However, despite tremendous efforts, it is still challenging to generate microbial strains that can produce target biochemical to high levels. Here, we report an example of biosynthesis of high-value and easy-recoverable derivatives built upon natural microbial pathways, leading to improvement in bioproduction efficiency. By leveraging pathways in solventogenic clostridia for co-producing acyl-CoAs, acids and alcohols as precursors, through rational screening for host strains and enzymes, systematic metabolic engineering-including elimination of putative prophages, we develop strains that can produce 20.3 g/L bu... More