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Syntrophy of Crypthecodinium cohnii and immobilized Zymomonas mobilis for docosahexaenoic acid production from sucrose-containing substrates

J Biotechnol. 2021-07; 
Inese Strazdina, Linards Klavins, Nina Galinina, Karlis Shvirksts, Mara Grube, Egils Stalidzans, Uldis Kalnenieks
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GenParts™ DNA Fragments … L33402) ORF with engineered AgeI restriction site inbetween, and BamHI and HindIII sites at its ends was delivered by GenScript. The DNA fragment was double-digested with BamHI and HindIII, and directionally cloned between the BamHI and HindIII restriction sites of the … Get A Quote

摘要

Marine heterotrophic dinoflagellate Crypthecodinium cohnii is an aerobic oleaginous microorganism that accumulates intracellular lipid with high content of 4,7,10,13,16,19-docosahexaenoic acid (DHA), a polyunsaturated ω-3 (22:6) fatty acid with multiple health benefits. C. cohnii can grow on glucose and ethanol, but not on sucrose or fructose. For conversion of sucrose-containing renewables to C. cohnii DHA, we investigated a syntrophic process, involving immobilized cells of ethanologenic bacterium Zymomonas mobilis for fermenting sucrose to ethanol. The non-respiring, NADH dehydrogenase-deficient Z. mobilis strain Zm6-ndh, with high ethanol yield both under anaerobic and aerobic conditions, was taken as the ... More

关键词

Crypthecodinium cohnii, Docosahexanoic acid, Immobilized cells, Levansucrase, Syntrophy, Zymomonas mobilis