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Custom Vector Construction> | … The heavy and light chain genes of the Fab 9D5 were synthesized (Genscript) and cloned into pFastBac Dual vector with the heavy chain under the polyhedrin promoter and the light chain under the P10 promoter with an N-terminal GP64 signal peptide on each chain. A TEV … | Get A Quote |
Norepinephrine is a biogenic amine neurotransmitter that has widespread effects on alertness, arousal and pain sensation. Consequently, blockers of norepinephrine uptake have served as vital tools to treat depression and chronic pain. Here, we employ the Drosophila melanogaster dopamine transporter as a surrogate for the norepinephrine transporter and determine X-ray structures of the transporter in its substrate-free and norepinephrine-bound forms. We also report structures of the transporter in complex with inhibitors of chronic pain including duloxetine, milnacipran and a synthetic opioid, tramadol. When compared to dopamine, we observe that norepinephrine binds in a different pose, in the vicinity of subsit... More