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Molecular cloning, characterization, and function analysis of the AMH gene in Yak (Bos grunniens) Sertoli cells

Theriogenology. 2021-01; 
Wenchang Qin, Bin Wang, Liuqing Yang, YuJie Yuan, Xianrong Xiong, Jian Li, Shi Yin
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DNA Sequencing … The products were cloned into a pMD™19-T vector (TaKaRa D10A2, Dalian, China) and transformed in competent E. coli DH5α cells (TaKaRa D9057A, Dalian, China). A positive clone was selected and subsequently sequenced by a company (Genscript Inc. Nanjing, China) … Get A Quote

摘要

Sertoli cells (SCs) are important testicular somatic cells that carry out various functions in spermatogenesis. Understanding the biological mechanisms underlying SC development may facilitate the understanding of animal reproduction. Anti-Mullerian hormone (AMH) is a dimeric glycoprotein produced by SCs and plays essential roles in spermatogenesis. In this study, we cloned the coding sequence of the yak AMH, predicated the structure of AMH protein, analyzed AMH expression in the testis at different stages, and studied the functions of AMH in yak SCs. The open reading frame (ORF) of the yak AMH contained 1728 bp and encoded 575 amino acids. Structural analysis revealed that the yak AMH protein had a highly cons... More

关键词

AMH, Gene cloning, Sertoli cell, Spermatogenesis, Yak