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Mutagenesis Services> | … The binding site of miR-19b-1-5p and ABL2 was verified: the wild type (WT) or mutant type (MUT) pmirGLO ABL2 were bought from GenScript Co., Ltd. (Nanjing, China). T24 and 5637 cells were seeded in 96-well plates. When … | Get A Quote |
background: Exosomes are involved in intercellular communication via specialized molecular cargo, such as microRNAs (miRNAs). However, the mechanisms underlying exosomal miR-19b-1-5p in bladder cancer remain largely unknown, thus, we aim to investigate the effect of exosomal miR-19b-1-5p on bladder cancer with the involvement of non-receptor protein tyrosine kinase Arg (ABL2). methods: miR-19b-1-5p and ABL2 expression were tested in bladder cancer. miR-19b-1-5p inhibition/elevation assays were conducted to determine its role in bladder cancer. Exosomes were extracted from bone marrow mesenchymal stem cells (BMSCs). Exosomes and T24 cells were co-cultured to verify their function in biological characteristics of... More