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Susceptibility of Well-Differentiated Airway Epithelial Cell Cultures from Domestic and Wild Animals to Severe Acute Respiratory Syndrome Coronavirus 2

Emerg Infect Dis. 2021-07; 
Mitra Gultom, Matthias Licheri, Laura Laloli, Manon Wider, Marina Strässle, Philip V'kovski, Silvio Steiner, Annika Kratzel, Tran Thi Nhu Thao, Lukas Probst, Hanspeter Stalder, Jasmine Portmann, Melle Holwerda, Nadine Ebert, Nadine Stokar-Regenscheit, Corinne Gurtner, Patrik Zanolari, Horst Posthaus, Simone Schuller, Amanda Vicente-Santos, Andres Moreira-Soto, Eugenia Corrales-Aguilar, Nicolas Ruggli, Gergely Tekes, Veronika von Messling, Bevan Sawatsky, Volker Thiel, Ronald Dijkman
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Molecular Biology Tools … Oklahoma/660/2013 strain (GenScript, https:// www.genscript.com) to detect IDV-infected cells. To … junctions. We counterstained all samples using DAPI (4',6-diamidino-2- phenylindole; Thermo Fisher Scientific) to visualize the nuclei … Get A Quote

摘要

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread globally, and the number of worldwide cases continues to rise. The zoonotic origins of SARS-CoV-2 and its intermediate and potential spillback host reservoirs, besides humans, remain largely unknown. Because of ethical and experimental constraints and more important, to reduce and refine animal experimentation, we used our repository of well-differentiated airway epithelial cell (AEC) cultures from various domesticated and wildlife animal species to assess their susceptibility to SARS-CoV-2. We observed that SARS-CoV-2 replicated efficiently only in monkey and cat AEC culture models. Whole-genome sequencing of progeny viruses revealed no ob... More

关键词

SARS-CoV-2, animal experimentation ethics, coronavirus disease, disease reservoirs, epidemics, epithelial cells, outbreaks, respiratory infections, severe acute respiratory syndrome 2, viruses, whole genome sequencing, zoonoses