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Molecular Biology Tools> | … Oklahoma/660/2013 strain (GenScript, https:// www.genscript.com) to detect IDV-infected cells. To … junctions. We counterstained all samples using DAPI (4',6-diamidino-2- phenylindole; Thermo Fisher Scientific) to visualize the nuclei … | Get A Quote |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread globally, and the number of worldwide cases continues to rise. The zoonotic origins of SARS-CoV-2 and its intermediate and potential spillback host reservoirs, besides humans, remain largely unknown. Because of ethical and experimental constraints and more important, to reduce and refine animal experimentation, we used our repository of well-differentiated airway epithelial cell (AEC) cultures from various domesticated and wildlife animal species to assess their susceptibility to SARS-CoV-2. We observed that SARS-CoV-2 replicated efficiently only in monkey and cat AEC culture models. Whole-genome sequencing of progeny viruses revealed no ob... More