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Enzymatic construction of shRNA library from oligonucleotide library

Genes Genomics. 2019-03; 
Seong Kyun Park, Yun Kee, Taehoon Ryu, Hyoki Kim, Byung Joon Hwang
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Oligo pool … Enzymatic construction of shRNA expression library. Step 1. Array synthesis of 88-mer oligonucleotides 88-mer oligonucleotides were synthesized by the CombiMatrix CustomArray ™ 90K platform(Custom array, Seattle, USA) … Get A Quote

摘要

background: Short hairpin RNAs (shRNAs) expressed from vectors have been used as an effective means of exploiting the RNA interference (RNAi) pathway in mammalian cells. Genome-scale screening with shRNA libraries has been used to investigate the relationship between genotypes and phenotypes on a large scale. Although several methods have been developed to construct shRNA libraries, their broad application has been limited by the high cost of constructing these libraries. objective: We develop a new method that efficiently constructs a shRNA library at low cost, using treatments with several enzymes and an oligonucleotide library. methods: The library of shRNA expression cassettes, which were cloned into a lent... More

关键词

High-throughput screen, Oligonucleotide library, RNAi, ShRNA library