| Products/Services Used | Details | Operation |
|---|---|---|
| Plasmid DNA Preparation | … coli DH5α cells (Tiangen). The recombinant plasmids were extracted using the AXGEN Plasmid Mini Kit (AXGEN, China) and sequenced by Genscript (Genscript Biotech Corpora- tion, Nanjing, China). The concentration of … | Get A Quote |
In this study, we developed a SYBR Green I real-time PCR method for the rapid and sensitive detection of novel porcine parvovirus 7 (PPV7). Specific primers were designed based on the highly conserved region within the Capsid gene of PPV7. The established method was 1,000 times more sensitive than the conventional PCR method and had a detection limit of 35.6 copies. This method was specific and had no cross-reactions with PCV2, PCV3, PRV, PEDV, PPV1, and PPV6. Experiments testing the intra and interassay precision demonstrated a high reproducibility. Testing the newly established method with 200 clinical samples revealed a detection rate up to 17.5% higher than that of the conventional PCR assay. The establishe... More
