| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | … cloning of the four DsU6 promoters into a pCFD5-gRNA-white vector, we entrusted a biotech company (Genscript, nanjing, china) to synthesize the four DsU6 promoter and replaced the DmU6:3 promoter that located in the plasmid of pCFD5-gRNA-white. Thus the four … | Get A Quote |
The invasive spotted-wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) has caused serious economic losses to the fruit industry. The conventional control methods have many limitations and genetic engineering technologies such as CRISPR/Cas9-mediated gene drive are promising approaches. In the CRISPR/Cas9 system, the transcriptional regulatory elements play an important role in the activities of gRNA. Thus, in order to improve the genome editing efficiency of the CRISPR/Cas9 system in D. suzukii, we cloned and tested four endogenous U6 promoters to drive mutagenesis of the white gene. Our results showed that all the four promoters could be used with variable efficiency. The promoter DsU6-3... More
