Products/Services Used | Details | Operation |
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PCR and Cloning> | … An SYBR premix ex taq II kit was purchased from Takara Biotechnology (Dalian, China). Primers were designed by GenScript China, Ltd (Nanjing, China) … Tissues were incubated with 3% H 2 O 2 (Sangon Biotech Co. Ltd, Shanghai, China) for 10 min … | Get A Quote |
A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice. However, whether it influences Th17 and regulatory T (Treg) cells as well as gut ecology remains unknown. In the present study, the intestinal injury mouse model was also established by intraperitoneal injection of cyclophosphamide (Cy) for three consecutive days. NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt). The levels of transforming growth factor (TGF)-β3 and transcription factor ... More