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Clinical Evaluation of a Real-Time PCR Assay for Simultaneous Detection of and Genotypic Markers of Clarithromycin Resistance Directly from Stool

J Clin Microbiol. 2021-02; 
Rebecca Marrero Rolon, Scott A Cunningham, Jayawant N Mandrekar, Erin T Polo, Robin Patel
Products/Services Used Details Operation
DNA Sequencing … 138 Positive samples underwent bi-directional Sanger sequencing (GenScript, Piscataway, NJ) to 139 assess the ability of the PCR assay to accurately detect the targeted SNPs associated with 140 clarithromycin resistance … Get A Quote

摘要

infection is mainly diagnosed non-invasively, with susceptibility testing traditionally requiring endoscopy. Treatment is empiric, with clarithromycin triple therapy recommended where resistance rates are below 15%. Rising clarithromycin resistance resulting in high therapy failure rates is seen worldwide but United States data is limited. We developed a real-time PCR assay for simultaneous detection of and genotypic markers of clarithromycin resistance directly from stool specimens. The assay was validated by testing 524 stool samples using an stool antigen test as the reference method for detection accuracy and Sanger sequencing to confirm genotypic susceptibility results. A separate set of 223 antigen posi... More

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