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Sphingolipid long-chain base hydroxylation influences plant growth and callose deposition in Physcomitrium patens

New Phytol. 2021-03; 
Jasmin Gömann, Cornelia Herrfurth, Agnieszka Zienkiewicz, Till Ischebeck, Tegan M Haslam, Ellen Hornung, Ivo Feussner
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PCR Cloning and Subcloning … (Genscript, Piscataway Township, New Jersey, USA) and cloned into the pYES2-CT vector (Thermo Fisher Scientific, Waltham, Massachusetts, USA) using the primers listed in Table S5. Successful cloning was confirmed by sequencing … Get A Quote

摘要

Sphingolipids are enriched in microdomains in the plant plasma membrane (PM). Hydroxyl groups in the characteristic long-chain base (LCB) moiety might be essential for the interaction between sphingolipids and sterols during microdomain formation. Investigating LCB hydroxylase mutants in Physcomitrium patens might therefore reveal the role of certain plant sphingolipids in the formation of PM subdomains. Physcomitrium patens mutants for the LCB C-4 hydroxylase S4H were generated by homologous recombination. Plants were characterised by analysing their sphingolipid and glycosylated sterol (SG) profiles and by investigating different gametophyte stages. s4h mutants lost the hydroxyl group at the C-4 position of ... More

关键词

Physcomitrium patens, LCB hydroxylation, callose, long-chain base (LCB) C-4 hydroxylase, microdomain, non-vascular plants, plant development, sphingolipid metabolism