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High-Level Expression of Palmitoylated MPP1 Recombinant Protein in Mammalian Cells

Membranes (Basel). 2021-09; 
Agnieszka Chytła, Weronika Gajdzik-Nowak, Agnieszka Biernatowska, Aleksander F Sikorski, Aleksander Czogalla
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Custom Vector Construction … Please let us know what you think of our products and services. … Hygro-ACP_His-tag_MPP1_TAP-tag expression vector was constructed and synthesized commercially (GenScript). The … Get A Quote

摘要

Our recent studies have pointed to an important role of the MAGUK family member, MPP1, as a crucial molecule interacting with flotillins and involved in the lateral organization of the erythroid plasma membrane. The palmitoylation of MPP1 seems to be an important element in this process; however, studies on the direct effect of palmitoylation on protein-protein or protein-membrane interactions in vitro are still challenging due to the difficulties in obtaining functional post-translationally modified recombinant proteins and the lack of comprehensive protocols for the purification of palmitoylated proteins. In this work, we present an optimized approach for the high-yield overexpression and purification of palm... More

关键词

Acyl-RAC, HEK-293F, MPP1, membrane rafts, palmitoylation, purification, transfection