Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | … Please let us know what you think of our products and services. … Hygro-ACP_His-tag_MPP1_TAP-tag expression vector was constructed and synthesized commercially (GenScript). The … | Get A Quote |
Our recent studies have pointed to an important role of the MAGUK family member, MPP1, as a crucial molecule interacting with flotillins and involved in the lateral organization of the erythroid plasma membrane. The palmitoylation of MPP1 seems to be an important element in this process; however, studies on the direct effect of palmitoylation on protein-protein or protein-membrane interactions in vitro are still challenging due to the difficulties in obtaining functional post-translationally modified recombinant proteins and the lack of comprehensive protocols for the purification of palmitoylated proteins. In this work, we present an optimized approach for the high-yield overexpression and purification of palm... More