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Tomato brown rugose fruit virus resistance generated by quadruple knockout of homologs of TOBAMOVIRUS MULTIPLICATION1 in tomato

Plant Physiol. 2022-06; 
Masayuki Ishikawa, Tetsuya Yoshida, Momoko Matsuyama, Yusuke Kouzai, Akihito Kano, Kazuhiro Ishibashi
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PCR Cloning and Subcloning … : KT383474.1, nucleotides 6–9 were deleted), and a unique AgeI restriction site were chemically synthesized, assembled, and cloned in pUC57 (pToBRFV-A2, prepared by GenScript). … Get A Quote

摘要

Tomato brown rugose fruit virus (ToBRFV) is an emerging virus of the genus Tobamovirus. ToBRFV overcomes the tobamovirus resistance gene Tm-22 and is rapidly spreading worldwide. Genetic resources for ToBRFV resistance are urgently needed. Here, we show that clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9)-mediated targeted mutagenesis of four tomato (Solanum lycopersicum) homologs of TOBAMOVIRUS MULTIPLICATION1 (TOM1), an Arabidopsis (Arabidopsis thaliana) gene essential for tobamovirus multiplication, confers resistance to ToBRFV in tomato plants. Quadruple-mutant plants did not show detectable ToBRFV coat protein (CP) accumulation or obvious defects in growt... More

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