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Catalog Peptides> | … The protein was eluted by adding 300 μg/ml FLAG peptide (GenScript). Eluted proteins were dialyzed overnight against the buffer containing 10 mM MOPS, 300 mM NaCl, 0.1 mM EGTA… | Get A Quote |
Centrosome positioning is essential for their function. Typically, centrosomes are transported to various cellular locations through the interaction of centrosomal microtubules (MTs) with motor proteins anchored at the cortex or the nuclear surface. However, it remains unknown how centrioles migrate in cellular contexts in which they do not nucleate MTs. Here, we demonstrate that during interphase, inactive centrioles move directly along the interphase MT network as Kinesin-1 cargo. We identify Pericentrin-Like-Protein (PLP) as a novel Kinesin-1 interacting molecule essential for centriole motility. In vitro assays show that PLP directly interacts with the cargo binding domain of Kinesin-1, allowing PLP to migr... More