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DNA Sequencing> | … highlighted the exquisite specificity of human TCRs that target two T cell epitopes from SARS … Protein preparation. The sequencing of RLQ- and YLQ-specific TCRs from COVID-19 CPs was … (Supplementary Table 11) and cloned into the expression vector pET22b (GenScript) … | Get A Quote |
T cells play a vital role in combatting SARS-CoV-2 and forming long-term memory responses. Whereas extensive structural information is available on neutralizing antibodies against SARS-CoV-2, such information on SARS-CoV-2-specific T-cell receptors (TCRs) bound to their peptide-MHC targets is lacking. Here we determine the structures of a public and a private TCR from COVID-19 convalescent patients in complex with HLA-A2 and two SARS-CoV-2 spike protein epitopes (YLQ and RLQ). The structures reveal the basis for selection of particular TRAV and TRBV germline genes by the public but not the private TCR, and for the ability of the TCRs to recognize natural variants of RLQ but not YLQ. Neither TCR recognizes homol... More