Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | … cloned in frame with the Luc+ coding sequence (GenScript®, Netherland). All deletion of the … IGF-1R sequence (GenScript®) using the numbering from the NCBI NM_000875 sequence. … | Get A Quote |
Mechanisms of drug-tolerance remain poorly understood and have been linked to genomic but also to non-genomic processes. 5-fluorouracil (5-FU), the most widely used chemotherapy in oncology is associated with resistance. While prescribed as an inhibitor of DNA replication, 5-FU alters all RNA pathways. Here, we show that 5-FU treatment leads to the production of fluorinated ribosomes exhibiting altered translational activities. 5-FU is incorporated into ribosomal RNAs of mature ribosomes in cancer cell lines, colorectal xenografts, and human tumors. Fluorinated ribosomes appear to be functional, yet, they display a selective translational activity towards mRNAs depending on the nature of their 5'-untranslated ... More