Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … Plasmid constructs were synthesized and provided by GenScript GenScript Inc. N/A … using ACD/ChemSketch (Advanced Chemistry Development Inc., Canada) and converted into PDB … | Get A Quote |
Fungal infections are the leading cause of mortality by eukaryotic pathogens, with an estimated 150 million severe life-threatening cases and 1.7 million deaths reported annually. The rapid emergence of multidrug-resistant fungal isolates highlights the urgent need for new drugs with new mechanisms of action. In fungi, pantothenate phosphorylation, catalyzed by PanK enzyme, is the first step in the utilization of pantothenic acid and coenzyme A biosynthesis. In all fungi sequenced so far, this enzyme is encoded by a single PanK gene. Here, we report the crystal structure of a fungal PanK alone as well as with high-affinity inhibitors from a single chemotype identified through a high-throughput chemical screen. ... More