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Hydrogen-bonds mediate liquid-liquid phase separation of mussel derived adhesive peptides

Nat Commun. 2022-10; 
Qi Guo, Guijin Zou, Xuliang Qian, Shujun Chen, Huajian Gao, Jing Yu
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Catalog Peptides … After increasing ionic strength of the solution to 600 mM by injecting a calculated amount of 5 M … All variants of GK-16 peptides were purchased from Genscript in which all Y amino acids … Get A Quote

摘要

Marine mussels achieve strong underwater adhesion by depositing mussel foot proteins (Mfps) that form coacervates during the protein secretion. However, the molecular mechanisms that govern the phase separation behaviors of the Mfps are still not fully understood. Here, we report that GK-16*, a peptide derived from the primary adhesive protein Mfp-5, forms coacervate in seawater conditions. Molecular dynamics simulations combined with point mutation experiments demonstrate that Dopa- and Gly- mediated hydrogen-bonding interactions are essential in the coacervation process. The properties of GK-16* coacervates could be controlled by tuning the strength of the electrostatic and Dopa-mediated hydrogen bond interac... More

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