Products/Services Used | Details | Operation |
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Mutagenesis Services> | … GFP into constructs in combinations as indicated and injected into single-cell stage embryos. … the same location seen in other vertebrates (Genscript). Site-directed mutagenesis was … | Get A Quote |
South American and African weakly electric fish independently evolved electric organs from muscle. In both groups, a voltage-gated sodium channel gene independently lost expression from muscle and gained it in the electric organ, allowing the channel to become specialized for generating electric signals. It is unknown how this voltage-gated sodium channel gene is targeted to muscle in any vertebrate. We describe an enhancer that selectively targets sodium channel expression to muscle. Next, we demonstrate how the loss of this enhancer, but not trans-activating factors, drove the loss of sodium channel gene expression from muscle in South American electric fish. While this enhancer is also altered in African ele... More