Products/Services Used | Details | Operation |
---|---|---|
Mutant Libraries> | … codon-optimized and synthesized by Genscript (9). The plasmid … Either SARS-CoV-2 RBD WT or the N501Y mutant were … nM for the immobilized SARS-CoV-2 RBD WT was assessed at … | Get A Quote |
The seasonal nature in the outbreaks of respiratory viral infections with increased transmission during low temperatures has been well established. The current COVID-19 pandemic makes no exception, and temperature has been suggested to play a role on the viability and transmissibility of SARS-CoV-2. The receptor binding domain (RBD) of the Spike glycoprotein binds to the angiotensin-converting enzyme 2 (ACE2) to initiate viral fusion. Studying the effect of temperature on the receptor-Spike interaction, we observed a significant and stepwise increase in RBD-ACE2 affinity at low temperatures, resulting in slower dissociation kinetics. This translated into enhanced interaction of the full Spike to ACE2 receptor a... More