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Accelerated delivery of dsRNA in lepidopteran midgut cells by a Galanthus nivalis lectin (GNA)-dsRNA-binding domain fusion protein

Pestic Biochem Physiol. 2021-04; 
Zarel Martinez, Kristof De Schutter, Els J M Van Damme, Elise Vogel, Niels Wynant, Jozef Vanden Broeck, Olivier Christiaens, Guy Smagghe
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摘要

Lepidopteran insects are highly refractory to oral RNA interference (RNAi). Degradation, impaired cellular uptake and intracellular transport of double-stranded RNA (dsRNA) are considered the major factors responsible for the reduced RNAi efficiency in these insects. In this study, the potential of lectins to improve dsRNA delivery and RNAi efficacy was evaluated. First, a fusion protein consisting of the Galanthus nivalis agglutinin (GNA) and a dsRNA binding domain was developed, further referred to as GNA:dsRBD (GNAF). Then, its ability to increase dsRNA uptake and transfection efficiency in lepidopteran midgut cells was evaluated, as well as its ability to protect and promote the RNAi response in the beet ar... More

关键词

Cellular uptake, GNA, Insects, Lectin, RNAi, dsRNA carrier, dsRNA delivery