Products/Services Used | Details | Operation |
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ORF cDNA Clones/MolecularCloud> | … ASD-risk genes open reading frames (ORFs) were purchased from Addgene and Genscript or amplified from human adult and fetal brain RNA (Takara) (see Supplementary Table 10) … | Get A Quote |
There are hundreds of risk genes associated with autism spectrum disorder (ASD), but signaling networks at the protein level remain unexplored. We use neuron-specific proximity-labeling proteomics (BioID2) to identify protein-protein interaction (PPI) networks for 41 ASD risk genes. Neuron-specific PPI networks, including synaptic transmission proteins, are disrupted by de novo missense variants. The PPI network map reveals convergent pathways, including mitochondrial/metabolic processes, Wnt signaling, and MAPK signaling. CRISPR knockout displays an association between mitochondrial activity and ASD risk genes. The PPI network shows an enrichment of 112 additional ASD risk genes and differentially expressed ge... More