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GenParts™ DNA Fragments> | … The fragment encoding the transcription terminators and MCS were synthesized by GenScript. Further derivatives of these plasmids were created using a combination of methods: … | Get A Quote |
is an important industrial microorganism and a widely used model object for research in the field of lactic acid bacteria (LAB) biology. The development of new and related LAB strains with improved properties, including phage-resistant strains for dairy fermentation, LAB-based vaccines or strains with altered genotypes for research purposes, are hindered by the lack of genome-editing tools that allow for the easy and straightforward incorporation of a significant amount of the novel genetic material, such as large genes or operons, into the chromosomes of these bacteria. We recently employed a suggested system based on the CRISPR-Cas-associated transposon for the editing of the genome. After the in-depth rede... More