Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … eGFP vector was purchased from GenScript. The EGFP-tagged VEGFA gene was excised from the original plasmid using EcoRI and XbaI Fast Digest restriction enzymes (Thermo … | Get A Quote |
Hydrogel wound dressings are effective in their ability to provide a wound-healing environment but are limited by their ability to promote later stages of revascularization. Here, a biosafe recombinant baculovirus expressing VEGFA tagged with EGFP is encapsulated in chitosan-coated alginate hydrogels using ionic cross-linking. The VEGFA, delivered by the baculovirus, significantly improves cell migration and angiogenesis to assist with the wound-healing process and revascularization. Moreover, the hydrogels have an encapsulation efficiency of 99.9%, no cytotoxicity, antimicrobial properties, good blood compatibility, promote hemostasis, and enable sustained delivery of baculoviruses over eight days. These hydro... More