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Lipid-Binding Aegerolysin from Biocontrol Fungus

Toxins (Basel). 2021-11; 
Nada Kraševec, Anastasija Panevska, Špela Lemež, Jaka Razinger, Kristina Sepčić, Gregor Anderluh, Marjetka Podobnik
Products/Services Used Details Operation
Bacterial Expression The synthetic construct encoding beauveriolysin blyA (GenScript, Piscataway, NJ, USA) was optimised for expression in E. coli. Get A Quote

摘要

Fungi are the most common pathogens of insects and thus important regulators of their populations. Lipid-binding aegerolysin proteins, which are commonly found in the fungal kingdom, may be involved in several biologically relevant processes including attack and defense against other organisms. Aegerolysins act alone or together with membrane-attack-complex/perforin (MACPF)-like proteins to form transmembrane pores that lead to cell lysis. We performed an in-depth bioinformatics analysis of aegerolysins in entomopathogenic fungi and selected a candidate aegerolysin, beauveriolysin A (BlyA) from . BlyA was expressed as a recombinant protein in , and purified to further determine its functional and structural pro... More

关键词

beauveriolysin, bioinformatics, ceramide phosphoethanolamine (CPE), entomopathogenic and antagonistic fungi, lipid binding, membrane-attack-complex/perforin (MACPF) protein, pore-forming proteins