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in Swiss and Dutch Guinea Pigs-Occurrence and Genetic Diversity

Pathogens. 2021-09; 
Silvia Ciuria, Michael S M Brouwer, Marende M de Gier, Yvonne van Zeeland, Alex Bossers, Barbara Prähauser, Julia Schädler, Jean-Michel Hatt, Marloes Heijne, Nicole Borel
Products/Services Used Details Operation
Custom DNA/RNA Oligos  All Dutch primers and probes were purchased from Eurogentec, Life Technologies Europe, Genscript, Biolegio Get A Quote

摘要

is a known pathogen in guinea pigs, causing conjunctivitis, respiratory infections and abortions. Recently, a -induced zoonotic link was identified as the etiology of severe community-acquired pneumonia in humans. Here, 784 conjunctival and rectal swabs originating from 260 guinea pigs and 110 rabbits from 64 husbandries in Switzerland, as well as 200 composite conjunctival swabs originating from 878 guinea pigs from 37 husbandries in The Netherlands were examined by real-time PCR followed by conventional PCR and sequencing. were detected in 2.3% (18/784) and 12.5% (25/200) of all Swiss and Dutch samples, respectively. An overall occurrence was detected in 2.7% (7/260) and 8.9% (78/878) of all Swiss and Dutch... More

关键词

Cavia porcellus, Chlamydia caviae, Chlamydia psittaci, Chlamydiaceae, Oryctolagus cuniculus, genetic diversity, ompA genotyping, zoonotic potential