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Evaluation of bacterial hosts for conversion of lignin-derived p-coumaric acid to 4-vinylphenol

Microb Cell Fact. 2021-09; 
Alberto Rodriguez, Jamie A Meadows, Ning Sun, Blake A Simmons, John M Gladden
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Bacterial Expression The gene coding for a phenolic acid decarboxylase (PAD) enzyme in B. amyloliquefaciens was codon-optimized for B. subtilis, C. glutamicum, and E. coli, and synthesized by Genscript (Piscataway, NJ, USA) Get A Quote

摘要

Hydroxycinnamic acids such as p-coumaric acid (CA) are chemically linked to lignin in grassy biomass with fairly labile ester bonds and therefore represent a straightforward opportunity to extract and valorize lignin components. In this work, we investigated the enzymatic conversion of CA extracted from lignocellulose to 4-vinylphenol (4VP) by expressing a microbial phenolic acid decarboxylase in Corynebacterium glutamicum, Escherichia coli, and Bacillus subtilis. The performance of the recombinant strains was evaluated in response to the substrate concentration in rich medium or a lignin liquor and the addition of an organic overlay to perform a continuous product extraction in batch cultures. We found that us... More

关键词

4-hydroxystyrene, 4-vinylphenol, Coumaric acid, Lignin, Microbial conversion