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Structure and function of aerotolerant, multiple-turnover THI4 thiazole synthases

Biochem J. 2021-09; 
Jaya Joshi, Qiang Li, Jorge D García-García, Bryan J Leong, You Hu, Steven D Bruner, Andrew D Hanson
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Bacterial Expression Selected THI4 genes were recoded for expression in E. coli or yeast and synthesized by GenScript (Piscataway, NJ) Get A Quote

摘要

Plant and fungal THI4 thiazole synthases produce the thiamin thiazole moiety in aerobic conditions via a single-turnover suicide reaction that uses an active-site Cys residue as sulfur donor. Multiple-turnover (i.e. catalytic) THI4s lacking an active-site Cys (non-Cys THI4s) that use sulfide as sulfur donor have been biochemically characterized -- but only from archaeal methanogens that are anaerobic, O2-sensitive hyperthermophiles from sulfide-rich habitats. These THI4s prefer iron as cofactor. A survey of prokaryote genomes uncovered non-Cys THI4s in aerobic mesophiles from sulfide-poor habitats, suggesting that multiple-turnover THI4 operation is possible in aerobic, mild, low-sulfide conditions. This was co... More

关键词

comparative genomics, suicide enzyme, thiamin, thiazole biosynthesis