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Design and Selection of Engineered Lytic Proteins With Decolonizing Activity

Front Microbiol. 2021-09; 
Diana Gutiérrez, Lorena Rodríguez-Rubio, Patricia Ruas-Madiedo, Lucía Fernández, Ana Belén Campelo, Yves Briers, Martin Weiss Nielsen, Karl Pedersen, Rob Lavigne, Pilar García, Ana Rodríguez
Products/Services Used Details Operation
Custom Vector Construction The optimized sequences were synthetized and cloned into the pUC57 vector by GenScript (Township, NJ, United States). Get A Quote

摘要

causes various infections in humans and animals, the skin being the principal reservoir of this pathogen. The widespread occurrence of methicillin-resistant (MRSA) limits the elimination and treatment of this pathogen. Phage lytic proteins have been proven as efficient antimicrobials against . Here, a set of 12 engineered proteins based on endolysins were conceptualized to select the most optimal following a stepwise funnel approach assessing parameters including turbidity reduction, minimum inhibitory concentration (MIC), time-kill curves, and antibiofilm assays, as well as testing their stability in a broad range of storage conditions (pH, temperature, and ionic strength). The engineered phage lysins LysRODI... More

关键词

Staphylococcus aureus, antimicrobial, endolysin, protein engineering, skin decontamination