Products/Services Used | Details | Operation |
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Bacterial Expression | NS1 from influenza A/PR8/34 was synthesised (GenScript, Piscataway, NJ, USA) and subcloned into the pCOLD bacterial expression vector (Takara, San Jose, CA, USA) with an N-terminal His6 fusion tag or the pcDNA3.1 vector (Thermo Fisher Scientific, Waltham, MA, USA) with a myc tag. | Get A Quote |
The cytoplasmic retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) initiate interferon (IFN) production and antiviral gene expression in response to RNA virus infection. Consequently, RLR signalling is tightly regulated by both host and viral factors. Tripartite motif protein 25 (TRIM25) is an E3 ligase that ubiquitinates multiple substrates within the RLR signalling cascade, playing both ubiquitination-dependent and -independent roles in RIG-I-mediated IFN induction. However, additional regulatory roles are emerging. Here, we show a novel interaction between TRIM25 and another protein in the RLR pathway that is essential for type I IFN induction, DEAD-box helicase 3X (DDX3X). In vitro assays and knoc... More