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Three-layered control of mRNA poly(A) tail synthesis in

Genes Dev. 2021-08; 
Matti Turtola, M Cemre Manav, Ananthanarayanan Kumar, Agnieszka Tudek, Seweryn Mroczek, Paweł S Krawczyk, Andrzej Dziembowski, Manfred Schmid, Lori A Passmore, Ana Casañal, Torben Heick Jensen
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Bacterial Expression A DNA sequence encoding full-length Pab1p from S. cerevisiae was codon-optimized for E. coli (GenScript Get A Quote

摘要

Biogenesis of most eukaryotic mRNAs involves the addition of an untemplated polyadenosine (pA) tail by the cleavage and polyadenylation machinery. The pA tail, and its exact length, impacts mRNA stability, nuclear export, and translation. To define how polyadenylation is controlled in , we have used an in vivo assay capable of assessing nuclear pA tail synthesis, analyzed tail length distributions by direct RNA sequencing, and reconstituted polyadenylation reactions with purified components. This revealed three control mechanisms for pA tail length. First, we found that the pA binding protein (PABP) Nab2p is the primary regulator of pA tail length. Second, when Nab2p is limiting, the nuclear pool of Pab1p, the ... More

关键词

Nab2p, Pab1p, cleavage and polyadenylation factor, mRNA nuclear export, poly(A) binding protein, poly(A) tail length, polyadenylation