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Barrier-to-autointegration-factor (Banf1) modulates DNA double-strand break repair pathway choice via regulation of DNA-dependent kinase (DNA-PK) activity

Nucleic Acids Res. 2021-04; 
Joshua T Burgess, Chee Man Cheong, Amila Suraweera, Thais Sobanski, Sam Beard, Keyur Dave, Maddison Rose, Didier Boucher, Laura V Croft, Mark N Adams, Kenneth O'Byrne, Derek J Richard, Emma Bolderson
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Custom Vector Construction The Flag-Banf1 construct was synthesized by Genscript in the pcDNA3.1+N-DYK vector in the BamHI-XhoI cloning sites. Get A Quote

摘要

DNA repair pathways are essential to maintain the integrity of the genome and prevent cell death and tumourigenesis. Here, we show that the Barrier-to-Autointegration Factor (Banf1) protein has a role in the repair of DNA double-strand breaks. Banf1 is characterized as a nuclear envelope protein and mutations in Banf1 are associated with the severe premature aging syndrome, Néstor-Guillermo Progeria Syndrome. We have previously shown that Banf1 directly regulates the activity of PARP1 in the repair of oxidative DNA lesions. Here, we show that Banf1 also has a role in modulating DNA double-strand break repair through regulation of the DNA-dependent Protein Kinase catalytic subunit, DNA-PKcs. Specifically, we de... More

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