至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Ectopic Expression of and Inactivation of Have a Synergistic Effect on Oil Accumulation in Plant Leaves

Plants (Basel). 2021-03; 
Zhiyang Zhai, Hui Liu, John Shanklin
Products/Services Used Details Operation
PCR Cloning and Subcloning Cys-OLE1 attained as a custom synthesized sequence (GenScript, Piscataway, NJ, USA) was amplified and cloned into pCHF3 between KpnI and XbaI. Get A Quote

摘要

During the transformation of wild-type (WT) , a T-DNA containing () was inserted by happenstance within the gene, resulting in significant reduction in amylose and increase in leaf oil content in the transgenic line (OG). The synergistic effect on oil accumulation of combining with the expression of was confirmed by transforming an independent mutant (GABI_914G01) with The resulting / transgenic lines showed higher leaf oil content than the individual WT or single mutant lines. Further stacking of the lipogenic factors WRINKLED1, Diacylglycerol O-Acyltransferase (DGAT1), and Cys-OLEOSIN1 (an engineered sesame OLEOSIN1) in significantly elevated its oil content in mature leaves to 2.3% of dry weight, whi... More

关键词

fatty acids, granule bound starch synthase, metabolic engineering, oleosin, triacylglycerol