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Transportome-wide engineering of Saccharomyces cerevisiae

Metab Eng. 2021-01; 
Guokun Wang, Iben Møller-Hansen, Mahsa Babaei, Vasil D'Ambrosio, Hanne Bjerre Christensen, Behrooz Darbani, Michael Krogh Jensen, Irina Borodina
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GenParts™ DNA Fragments Synthesized DNA fragments were utilized for ADE2 disruption optimization (Genscript or Twist Bioscience Get A Quote

摘要

Synthetic biology enables the production of small molecules by recombinant microbes for pharma, food, and materials applications. The secretion of products reduces the cost of separation and purification, but it is challenging to engineer due to the limited understanding of the transporter proteins' functions. Here we describe a method for genome-wide transporter disruption that, in combination with a metabolite biosensor, enables the identification of transporters impacting the production of a given target metabolite in yeast Saccharomyces cerevisiae. We applied the method to study the transport of xenobiotic compounds, cis,cis-muconic acid (CCM), protocatechuic acid (PCA), and betaxanthins. We found 22 transp... More

关键词

Betaxanthins, Cell factory, Metabolic engineering, Muconic acid, Protocatechuic acid, Transporter protein