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A highly conserved glutamic acid in ALFY inhibits membrane binding to aid in aggregate clearance

Traffic. 2020-12; 
Erin F Reinhart, Nicole A Litt, Sarah Katzenell, Maria Pellegrini, Ai Yamamoto, Michael J Ragusa
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PCR Cloning and Subcloning after the first and second copy of EEA1-FYVE was synthesized with a His12 GST TEV sequence and cloned into pET-24a(+) using BamHI and XhoI by GenScript Get A Quote

摘要

Autophagy-linked FYVE protein (ALFY) is a large, multidomain protein involved in the degradation of protein aggregates by selective autophagy. The C-terminal FYVE domain of ALFY has been shown to bind phosphatidylinositol 3-phosphate (PI(3)P); however, ALFY only partially colocalizes with other FYVE domains in cells. Thus, we asked if the FYVE domain of ALFY has distinct membrane binding properties compared to other FYVE domains and whether these properties might affect its function in vivo. We found that the FYVE domain of ALFY binds weakly to PI(3)P containing membranes in vitro. This weak binding is the result of a highly conserved glutamic acid within the membrane insertion loop in the FYVE domain of ALFY t... More

关键词

ALFY, FYVE domain, autophagy, nuclear magnetic resonance spectroscopy, phosphatidylinositol 3-phosphate, protein structure