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Construction and characterization of two SARS-CoV-2 minigenome replicon systems

J Med Virol. 2022-02; 
Hu Zhang, Douglas K Fischer, Masahiro Shuda, Patrick S Moore, Shou-Jiang Gao, Zandrea Ambrose, Haitao Guo
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ORF cDNA Clones/MolecularCloud … resistance gene (BSR) fusion gene ORF (Lb) and SARS-CoV-2 N ORF in tandem was partially synthesized by Genscript and assembled with a NLuc-containing PCR … Get A Quote

摘要

The ongoing COVID-19 pandemic severely impacts global public health and economies. To facilitate research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virology and antiviral discovery, a noninfectious viral replicon system operating under biosafety level 2 containment is warranted. We report herein the construction and characterization of two SARS-CoV-2 minigenome replicon systems. First, we constructed the IVT-CoV2-Rep complementary DNA template to generate a replicon messenger RNA (mRNA) with nanoluciferase (NLuc) reporter via in vitro transcription (IVT). The replicon mRNA transfection assay demonstrated a rapid and transient replication of IVT-CoV2-Rep in a variety of cell lines, which c... More

关键词

SARS coronavirus, antiviral agents, cellular effect, disease control, immune responses, innate immunity, mRNA/splicing, virus classification