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Characterization of a Cleavable Fusion of Human CYP24A1 with Adrenodoxin Reveals the Variable Role of Hydrophobics in Redox Partner Binding

Biochemistry. 2022-01; 
Natalie Jay, Sean R Duffy, D Fernando Estrada
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Mammalian Expression … underlined TEV recognition sequence (HRHQPVTIGEPAENLYFQGENLYFQG), and the mature form of human CYP24A1 (UniProt ID Q07973) was synthesized (GenScript) and cloned … Get A Quote

摘要

The improper maintenance of the bioactivated form of vitamin-D (1α,25(OH)D) may result in vitamin-D insufficiency and therefore compromise the absorption of dietary calcium. A significant regulator of vitamin-D metabolism is the inactivating function of the mitochondrial enzyme cytochrome P450 24A1 (CYP24A1). In humans, CYP24A1 carries out hydroxylation of carbon-23 (C23) or carbon-24 (C24) of the 1α,25(OH)D side chain, eventually resulting in production of either an antagonist of the vitamin-D receptor (C23 pathway) or calcitroic acid (C24 pathway). Despite its importance to human health, the human isoform (hCYP24A1) remains largely uncharacterized due in part to the difficulty in producing the enzyme using ... More

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