Products/Services Used | Details | Operation |
---|---|---|
Custom DNA/RNA Oligos> | … (35) Briefly, SELP genes were built through the assembly of oligos (synthetized by Genscript Biotech, Piscataway, New Jersey), subsequently cloned into a pET-29b(+) expression … | Get A Quote |
The modulation of reaction kinetics with horseradish peroxidase (HRP)-catalyzed cross-linking of proteins remains a useful strategy to modulate hydrogel formation. Here, we demonstrate that the presence of positively charged lysines in silk-elastin-like polymers impacts the thermal transition temperature of these proteins, while the location in the primary sequence modulates the reactivity of the tyrosines. The positively charged lysine side chains decreased π-π interactions among the tyrosines and reduced the rate of formation and number of HRP-mediated dityrosine bonds, dependent on the proximity of the charged group to the tyrosine. The results suggest that the location of repulsive charges can be used to ... More