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Antibody Modification and Purification Services> | ...subtracted. As a positive control for the assay Hek-Blue cells were incubated with various doses of purified interferon alpha 2b (GenScript, NJ, USA). RESULTS AND DISCUSSION Tuning duplex binding affinity with combinations of modifications We and others... | Get A Quote |
We report that combining a DNA analog (2'F-ANA) with rigid RNA analogs [2'F-RNA and/or locked nucleic acid (LNA)] in siRNA duplexes can produce gene silencing agents with enhanced potency. The favored conformations of these two analogs are different, and combining them in a 1-1 pattern led to reduced affinity, whereas alternating short continuous regions of individual modifications increased affinity relative to an RNA:RNA duplex. Thus, the binding affinity at key regions of the siRNA duplex could be tuned by changing the pattern of incorporation of DNA-like and RNA-like nucleotides. These heavily or fully modified duplexes are active against a range of mRNA targets. Effective patterns of modification... More