至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Intein splicing efficiency and RadA levels can control the mode of archaeal DNA replication

SCIENCE ADVANCES. 2024-09; 
Geraldy L S Liman , Christopher W Lennon , Jaylin L Mandley , Alina M Galyon , Kelly M Zatopek , Andrew F Gardner , Thomas J Santangelo
Products/Services Used Details Operation
Gene Synthesis For native extein splicing assays with Tk-RadA-inteinΔ286–585 , this construct was commercially synthesized and subcloned into the pET45b(+) vector backbone in-frame with an N-terminal His tag (GenScript, USA). Get A Quote
Mutagenesis Services Get A Quote

摘要

Inteins (intervening proteins), mobile genetic elements removed through protein splicing, often interrupt proteins required for DNA replication, recombination, and repair. An abundance of in vitro evidence implies that inteins may act as regulatory elements, whereby reduced splicing inhibits production of the mature protein lacking the intein, but in vivo evidence of regulatory intein excision in the native host is absent. The model archaeon Thermococcus kodakarensis encodes 15 inteins, and we establish the impacts of intein splicing inhibition on host physiology and replication in vivo. We report that a decrease in intein splicing efficiency of the recombinase RadA, a Rad51/RecA homolog, has widespread physiol... More

关键词