Monkeypox virus

E.coli

His	E8L (Met1-Thr275) Accession # QJQ40248.1
N-term	C-term

Cell surface-binding protein; Carbonic anhydrase homolog

Mouse

E.coli

His	SP17 (Met1-Asn149)_x000D_ Accession # Q62252
N-term	C-term

CT22; Sp17-1; Sperm protein 17; SPA17;

Monkeypox virus

HEK293

His	A30 (Gln22-Leu146)_x000D_ Accession # Q8V4U9
N-term	C-term

Envelope protein A28 homolog; Protein A30; A30L; A30

Broad Multi Color Pre-Stained Protein Standard is designed for observing protein separation during SDS-PAGE, verifying western transfer efficiency on membranes, and approximating the size of proteins.

sELISA; WB;

Mouse IgG1,κ

Biotin

purified recombinant Protein A

Human

HEK293

TIGIT (Met22-Pro141)_x000D_ Accession # Q495A1-1	hFc
N-term	C-term

TIGIT; VSIG9; VSTM3; WUCAM

Mouse

HEK293

TIGIT (Gly26-Thr143)_x000D_ Accession # NP_001139797.1	hFc
N-term	C-term

TIGIT; VSIG9; VSTM3; WUCAM

Cynomolgus

HEK293

TIM-4 (Glu25-Gln305) Accession # XP_005558436.2	His
N-term	C-term

TIMD-4; TIMD4; TIM-4;

Human

HEK 293

Mesothelin (Glu296-Gly580) Accession # Q13421	hFc
N-term	C-term

MSLN; CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein

GenScript’s Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens.The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF.Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.