| Products/Services Used | Details | Operation |
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| Catalog Products | ... Expi293 cells were transfected using Expifectamine transfection reagent (Invitrogen). After 7 days, the culture media were collected, and the fusion proteins were purified using affinity chromatography with Protein A Sepharose (GenScript, Piscataway, NJ, USA). ... | Get A Quote |
Heat shock protein 70-1A (HSP70-1A) is a stress-inducible protein that provides an essential intracellular molecular chaperone function; however, the mechanism of HSP70-1A in angiogenesis has not been clarified. Herein, HSP70-1A gene silencing implicated this protein in angiogenesis. Additionally, recombinant human HSP70-1A (rhHSP70-1A) was able to stimulate human umbilical vein endothelial cell (HUVEC) migration and tube formation in vitro and microvessel formation in vivo similarly to recombinant human vascular endothelial growth factor (rhVEGF). Furthermore, rhHSP70-1A was tightly bound to the surface of HUVECs and participated in extracellular signal-related kinase (ERK)-dependent angiogenesis. Together, ... More
