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Constitutive expression Of NGF And P75(NTR) affected by bladder distension and NGF antisense treatment.

Life Sci.. 2016-03; 
Kashyap M, Pore S, Yoshimura N, Tyagi P.
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Catalog Products ... Experiments involving bladder localization used ODN with a 5′ tag of TYE™ 563. Similar length scrambled sequence 5′ACGACCTCGCGACCGGCC3′ was designed using web-based tool https://www.genscript.com/ssl-bin/app/scramble. ... Get A Quote

摘要

AIMS: It is known that bladder exposure to noxious stimuli elicits nerve growth factor (NGF) expression with region wise differences. Here, we investigated the effect of bladder distension (cystometry) and bladder wall injection of NGF antisense oligonucleotide (ODN) together as well as separately on spontaneous (constitutive) expression of NGF and its cognate p75 neurotrophin receptor (p75(NTR)). METHOD: Under isoflurane anesthesia, either 15μg of protamine sulfate (vehicle) alone or complexed with 1.5μg of NGF antisense or scrambled ODN was injected (10μL) at 4 sites in bladder wall of 24 adult female Sprague-Dawley rats and 6 rats were left untreated (n=30). Under urethane anesthesia, cystometry (CMG) was... More

关键词

Constitutive; NGF; Protamine; Reflex voiding; p75(NTR)