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Nol9 Is a Spatial Regulator for the Human ITS2 Pre-rRNA Endonuclease–Kinase Complex

JMB. 2019-07; 
JacobGordonMonica C.PillonRobin E.Stanley
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ORF cDNA Clones/MolecularCloud Homo sapiens Las1L and Nol9 constructs were generated using cDNA provided by GenScript. Get A Quote

摘要

The ribosome plays a universal role in translating the cellular proteome. Defects in the ribosome assembly factor Las1L are associated with congenital lethal motor neuron disease and X-linked intellectual disability disorders, yet its role in processing precursor ribosomal RNA (pre-rRNA) is largely unclear. The Las1L endoribonuclease associates with the Nol9 polynucleotide kinase to form the internal transcribed spacer 2 (ITS2) pre-rRNA endonuclease–kinase machinery. Together, Las1L–Nol9 catalyzes RNA cleavage and phosphorylation to mark the ITS2 for degradation. While ITS2 processing is critical for the production of functional ribosomes, the regulation of mammalian Las1L–Nol9 remains obscure. Here we ch... More

关键词

Las1LNol9endoribonucleasepolynucleotide kinasenucleolar localization sequence